SidaGhanaV1, Main, Exploration, bibRecord, 000C89

Quasispecies analysis of novel HIV‐1 recombinants of subtypes A and G reveals no similarity to the mosaic structure of CRF02_AG

Identifieur interne : 000C89 ( Main/Exploration ); précédent : 000C88; suivant : 000C90

Quasispecies analysis of novel HIV‐1 recombinants of subtypes A and G reveals no similarity to the mosaic structure of CRF02_AG

Auteurs : Rebecca L. R. Powell [États-Unis] ; Frank A. J. Konings [États-Unis] ; Aubin Nanfack [Cameroun] ; Sherri Burda [États-Unis] ; Mateusz M. Urbanski [États-Unis] ; D. Saa [Cameroun] ; Phillipe N. Nyambi [États-Unis]

Source :

Journal of Medical Virology [ 0146-6615 ] ; 2007-09.

RBID : ISTEX:EB49E7B8174AF945CCC0CBF0C7A881B69BC77A77

Descripteurs français

Wicri :
- geographic : Cameroun.

English descriptors

KwdEn :
- Bootscanning, Bootstrap, Bootstrap plots, Bootstrap value, Breakpoint, Breakpoint analysis, Breakpoints, Burda, Cameroon, Clone, Dual infections, Genetic material, Grant sponsor, Human virus type, Hxb2, Hxb2 location, Konings, Nyambi, Parental subtypes, Phylogenetic, Phylogenetic analysis, Phylogenetic trees, Present study, Primer, Quasispecies, Recombinant, Recombinant form, Recombinant forms, Recombinant pattern, Recombinant patterns, Recombination, Recombination breakpoints, Reference sequences, Replicative, Study subjects, Subsequent breakpoint analysis, Subtype, Subtypes, Tness, Urfs, Viral, Viral forms, Virol, Virus, Virus culture, Virus type, York university school, Zhong.
Teeft :
- Bootscanning, Bootstrap, Bootstrap plots, Bootstrap value, Breakpoint, Breakpoint analysis, Breakpoints, Burda, Cameroon, Clone, Dual infections, Genetic material, Grant sponsor, Human virus type, Hxb2, Hxb2 location, Konings, Nyambi, Parental subtypes, Phylogenetic, Phylogenetic analysis, Phylogenetic trees, Present study, Primer, Quasispecies, Recombinant, Recombinant form, Recombinant forms, Recombinant pattern, Recombinant patterns, Recombination, Recombination breakpoints, Reference sequences, Replicative, Study subjects, Subsequent breakpoint analysis, Subtype, Subtypes, Tness, Urfs, Viral, Viral forms, Virol, Virus, Virus culture, Virus type, York university school, Zhong.

Abstract

HIV‐1 circulating recombinant form (CRF) 02_AG is responsible for greater than 65% of HIV‐1 infections in Cameroon and is widespread across West and West‐Central Africa. The parental subtypes A1 and G cocirculate in this part of Africa, and high rates of infection predispose to the generation of AG unique recombinant forms (URFs). Little is known as to whether A1 and G can recombine and thrive in vivo with breakpoints other than those characteristic of CRF02_AG. In this study, six unique recombinant viruses of subtypes A1 and G were identified in two individuals in Cameroon. A 1.5 kb fragment of the reverse transcriptase (RT) region of pol (HXB2 location 2,612–4,159) and the entire env gene (HXB2 location 6,202–9,096) were evaluated by phylogenetic and breakpoint analyses. Each URF was found to have breakpoints different than CRF02_AG, indicating that A and G gene segments are functionally compatible with more than one pattern of recombination. Furthermore, contemporaneous, cultured viruses from these individuals were analyzed, revealing different proportions of URFs compared to those found in plasma, possibly indicating compart mentalization and/or phenotypic variation among the URFs. CRF02_AG emerged from West‐Central Africa to become a highly successful viral strain. As such, monitoring the spread of newly emerging AG recombinants is critical not only for understanding the epidemiology of HIV‐1, but also in the design of future therapeutics and vaccines appropriate to this part of Africa, and globally. J. Med. Virol. 79:1270–1285, 2007. © Wiley‐Liss, Inc.

Url:

https://api.istex.fr/document/EB49E7B8174AF945CCC0CBF0C7A881B69BC77A77/fulltext/pdf

DOI: 10.1002/jmv.20937

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">HIV‐1 circulating recombinant form (CRF) 02_AG is responsible for greater than 65% of HIV‐1 infections in Cameroon and is widespread across West and West‐Central Africa. The parental subtypes A1 and G cocirculate in this part of Africa, and high rates of infection predispose to the generation of AG unique recombinant forms (URFs). Little is known as to whether A1 and G can recombine and thrive in vivo with breakpoints other than those characteristic of CRF02_AG. In this study, six unique recombinant viruses of subtypes A1 and G were identified in two individuals in Cameroon. A 1.5 kb fragment of the reverse transcriptase (RT) region of pol (HXB2 location 2,612–4,159) and the entire env gene (HXB2 location 6,202–9,096) were evaluated by phylogenetic and breakpoint analyses. Each URF was found to have breakpoints different than CRF02_AG, indicating that A and G gene segments are functionally compatible with more than one pattern of recombination. Furthermore, contemporaneous, cultured viruses from these individuals were analyzed, revealing different proportions of URFs compared to those found in plasma, possibly indicating compart mentalization and/or phenotypic variation among the URFs. CRF02_AG emerged from West‐Central Africa to become a highly successful viral strain. As such, monitoring the spread of newly emerging AG recombinants is critical not only for understanding the epidemiology of HIV‐1, but also in the design of future therapeutics and vaccines appropriate to this part of Africa, and globally. J. Med. Virol. 79:1270–1285, 2007. © Wiley‐Liss, Inc.</div>
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Quasispecies analysis of novel HIV‐1 recombinants of subtypes A and G reveals no similarity to the mosaic structure of CRF02_AG

Quasispecies analysis of novel HIV‐1 recombinants of subtypes A and G reveals no similarity to the mosaic structure of CRF02_AG

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri